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CLC number: R657.5+1

On-line Access: 2014-06-07

Received: 2013-09-10

Revision Accepted: 2013-11-11

Crosschecked: 2014-05-20

Cited: 4

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.6 P.556-565


Clodronate-superparamagnetic iron oxide-containing liposomes attenuate renal injury in rats with severe acute pancreatitis*

Author(s):  Sheng-chun Dang, Yan-hua Zeng, Ping-jiang Wang, Bao-ding Chen, Rong-fang Chen, Arun Kumar Singh, Pankaj Kumar, Shu Feng, Lei Cui, Hao Wang, Jian-xin Zhang

Affiliation(s):  . Department of General Surgery, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China

Corresponding email(s):   dscgu@163.com

Key Words:  Pancreatitis, Clodronate disodium, Macrophage, Kidney injury

Sheng-chun Dang, Yan-hua Zeng, Ping-jiang Wang, Bao-ding Chen, Rong-fang Chen, Arun Kumar Singh, Pankaj Kumar, Shu Feng, Lei Cui, Hao Wang, Jian-xin Zhang. Clodronate-superparamagnetic iron oxide-containing liposomes attenuate renal injury in rats with severe acute pancreatitis[J]. Journal of Zhejiang University Science B, 2014, 15(6): 556-565.

@article{title="Clodronate-superparamagnetic iron oxide-containing liposomes attenuate renal injury in rats with severe acute pancreatitis",
author="Sheng-chun Dang, Yan-hua Zeng, Ping-jiang Wang, Bao-ding Chen, Rong-fang Chen, Arun Kumar Singh, Pankaj Kumar, Shu Feng, Lei Cui, Hao Wang, Jian-xin Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Clodronate-superparamagnetic iron oxide-containing liposomes attenuate renal injury in rats with severe acute pancreatitis
%A Sheng-chun Dang
%A Yan-hua Zeng
%A Ping-jiang Wang
%A Bao-ding Chen
%A Rong-fang Chen
%A Arun Kumar Singh
%A Pankaj Kumar
%A Shu Feng
%A Lei Cui
%A Hao Wang
%A Jian-xin Zhang
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 6
%P 556-565
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300244

T1 - Clodronate-superparamagnetic iron oxide-containing liposomes attenuate renal injury in rats with severe acute pancreatitis
A1 - Sheng-chun Dang
A1 - Yan-hua Zeng
A1 - Ping-jiang Wang
A1 - Bao-ding Chen
A1 - Rong-fang Chen
A1 - Arun Kumar Singh
A1 - Pankaj Kumar
A1 - Shu Feng
A1 - Lei Cui
A1 - Hao Wang
A1 - Jian-xin Zhang
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 6
SP - 556
EP - 565
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300244

Background and objective: It has been shown that macrophages play an important role in the development of severe acute pancreatitis (SAP), and eventually lead to multiple organ failure (MOF). Clodronate-liposome selectively depleted macrophages. This study was to investigate the role of renal macrophage infiltration in acute renal injury in rats with SAP and to evaluate the potential of superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance imaging (MRI) for diagnosis. Methods: Superparamagnetic Fe3O4 nanoparticles were prepared by chemical coprecipitation. SPIO-liposomes and SPIO-clodronate-liposomes were prepared by the thin film method. SAP models were prepared by injection of sodium taurocholate into the subcapsular space of rat pancreas. Sprague-Dawley rats were randomly divided into a control group, SAP plus SPIO-liposome (P) group, and SAP plus SPIO-clodronate-containing liposome (T) group. kidney injury was evaluated by T2-weighted MRI scan. The levels of serum amylase (SAM), blood urea nitrogen (BUN), and serum creatinine (SCr) were measured by an automated enzymatic method. Serum tumor necrosis factor-α (TNF-α) was measured by enzyme-linked immunosorbent assay (ELISA). Pathological changes in the pancreas and kidney were observed using hematoxylin and eosin (H&E) staining, while cell apoptosis was detected with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. In addition, the macrophage markers (CD68) of the renal tissue were detected with immunohistochemistry. Results: The pathological changes in the pancreas and kidneys of rats in the T group were milder than those in the P group. The MRI signal intensity of the kidneys in the P and T groups was significantly lower than that in the control group. There were significant changes in the two experimental groups (P<0.01). The levels of SAM, Bun, SCr, and TNF-α in rats in the P group were higher than those in the control group (P<0.01) and in the T group (P<0.01). The apoptosis of the kidney in the T group was higher than that in the P group at 2 and 6 h (P<0.01). Conclusions: Clodronate-containing liposomes protected against renal injury in SAP rats, and SPIO can be used as a tracer for MRI examination to detect renal injury in SAP rats. SPIO-aided MRI provided an efficient non-invasive way to monitor the migration of macrophages after renal injury in rats with SAP.


研究方法:采用胰腺被膜下均匀注射5%牛磺胆酸钠制作SAP模型。SD大鼠48只,随机分为对照组(C组)、空白SPIO脂质体组(P组)和氯屈膦酸二钠+SPIO脂质体组(T组)。P组和T组大鼠制作SAP模型。制模2 h和6 h后取肠系膜上静脉血液,检测各组大鼠血清中淀粉酶、尿素氮、血肌酐和肿瘤坏死因子-α的含量,观察胰腺及肾组织的病理学变化及进行病理评分,通过检测肾组织的TUNEL染色及CD68表达研究氯屈膦酸二钠-超顺磁性氧化铁脂质体对肾组织巨噬细胞凋亡的影响并进行MRI诊断。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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