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On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.12 P.969-976

http://doi.org/10.1631/jzus.B0820154


Antiviral activities of extracts from Hong Kong seaweeds


Author(s):  Hui WANG, Engchoon Vincent OOI, Put O. Ang Jr.

Affiliation(s):  Medical College, Jinan University, Guangzhou 510632, China; more

Corresponding email(s):   twanghzzm@jnu.edu.cn, put-ang@cuhk.edu.hk

Key Words:  Hong Kong seaweed, Hydroclathrus clathratus, Antiviral activity, Herpes simplex virus (HSV), Flow cytometry


Hui WANG, Engchoon Vincent OOI, Put O. Ang Jr.. Antiviral activities of extracts from Hong Kong seaweeds[J]. Journal of Zhejiang University Science B, 2008, 9(12): 969-976.

@article{title="Antiviral activities of extracts from Hong Kong seaweeds",
author="Hui WANG, Engchoon Vincent OOI, Put O. Ang Jr.",
journal="Journal of Zhejiang University Science B",
volume="9",
number="12",
pages="969-976",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820154"
}

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%T Antiviral activities of extracts from Hong Kong seaweeds
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%A Engchoon Vincent OOI
%A Put O. Ang Jr.
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820154

TY - JOUR
T1 - Antiviral activities of extracts from Hong Kong seaweeds
A1 - Hui WANG
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A1 - Put O. Ang Jr.
J0 - Journal of Zhejiang University Science B
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SP - 969
EP - 976
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820154


Abstract: 
We extracted six Hong Kong brown seaweed species with hot water for their antiviral properties. The cytotoxicity and antiviral activity of these extracts were tested by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenlytetrezolium bromide] method, cytopathic effect reduction assay, and plaque reduction assay. The antiviral effect was further determined by flow cytometric analysis. The results showed that most of these extracts inhibited the propagation of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) standard strains with very low cytotoxicity to the host cells. The extracts of Hydroclathrus clathratus and Lobophora variegata showed more potential anti-HSV activities than the extracts of the other four seaweeds. They also had moderate anti- respiratory syncytial virus (RSV) activities but could not inhibit influenza A virus. Hydroclathrus clathratus was further extracted by diluted acid and alkali and the antiviral effects of the extracts were also detected. The result showed that the hot water extract contained the main carbohydrate components that exhibited the antiviral activities against various strains of HSV, including the acyclovir-resistant strain. HI-3, a compound fractionated from this hot water extract, showed a dose-dependent anti-HSV activity in flow cytometric analysis and plaque reduction assay.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Ahn, M.J., Yoon, K.D., Kim, C.Y., Min, S.Y., Kim, Y., Kim, H.J., Kim, J.H., Shin, C.G., Lee, C.K., Kim, T.G., et al., 2002. Inhibition of HIV-1 reverse transcriptase and HIV-1 integrase and antiviral activity of Korean seaweed extracts. J. Appl. Phycol., 14(5):325-329.

[2] Alché, L.E., Barquero, A.A., Sanjuan, N.A., Coto, C.E., 2002. An antiviral principle present in a purified fraction from Melia Azedarach L. leaf aqueous extract restrains herpes simplex virus type-1 propagation. Phytother. Res., 16(4):348-352.

[3] Ammendolia, M.G., Marchetti, M., Superti, F., 2007. Bovine lactoferrin prevents the entry and intercellular spread of herpes simplex virus type 1 in Green Monkey Kidney cells. Antiviral Res., 76(3):252-262.

[4] Ang, P.O., 2005. Studies of Marine Algae in Hong Kong. In: Critichley, A.T., Ohno, M., Largo, D. (Eds.), World Seaweed Resources. ETI Information Services Ltd., Wokingham, Berkshire, UK, Part 3.04.

[5] Baba, M., Snoeck, R., Pauwels, R., de Clercq, E., 1988. Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus. Antimicrob. Agents Chemother., 32(11):1742-1745.

[6] Becker, Y., 2007. Past, present and future of RSV and PIV vaccines and anti-RSV antibodies for the protection of humans against RSV. Expert Opin. Ther. Pat., 17(8):941-953.

[7] Béress, A., Wassermann, O., Bruhn, T., Béress, L., 1993. A new procedure for the isolation of anti-HIV compounds (polysaccharides and polyphenols) from the marine alga Fucus vesiculosus. J. Nat. Prod., 56(4):478-488.

[8] Bourne, K.Z., Bourne, N., Reising, S.F., Stanberry, L.R., 1999. Plant products as topical microbicide candidates: assessment of in vitro and in vivo activity against herpes simplex virus type 2. Antiviral Res., 42(3):219-226.

[9] Burleson, F.G., Chambers, T.M., Wiedbrauk, D.L., 1992. Virology: A Laboratory Manual. Academic Press, California, San Diego, p.53-61.

[10] Chi-Ming Chiu, L., Zhu, W., Eng-Choon Ooi, V., 2004. A polysaccharide fraction from medicinal herb Prunella vulgaris downregulates the expression of herpes simplex virus antigen in Vero cells. J. Ethnopharmacol., 93(1):63-68.

[11] Chutkowski, C., Olson, B., McDonough, A., Mahoney, J., McSharry, J.J., 2002. Use of a single monoclonal antibody to determine the susceptibilities of herpes simplex virus type 1 and type 2 clinical isolates to acyclovir. Clin. Diagn. Lab. Immunol., 9(6):1379-1381.

[12] Damonte, E., Neyts, J., Pujol, C.A., Snoeck, R., Andrei, G., Ikeda, S., Witvrouw, M., Reymen, D., Haines, H., Matulewicz, M.C., et al., 1994. Antiviral activity of a sulphated polysaccharide from the red seaweed Nothogenia fastigiata. Biochem. Pharmacol., 47(12):2187-2192.

[13] de Clercq, E., 1985. Antiviral and antimetabolic activities of neplanocins. Antimicrob. Agents Chemother., 28(1):84-89.

[14] de Clercq, E., 1996. Perspectives for the chemotherapy of respiratory syncytial virus (RSV) infections. Int. J. Antimicrob. Agents, 7(3):193-202.

[15] González, M.E., Alarcón, B., Carrasco, L., 1987. Polysaccharides as antiviral agents: antiviral activity of carrageenan. Antimicrob. Agents Chemother., 31(9):1388-1393.

[16] Huleihel, M., Ishanu, V., Tal, J., Arad, S., 2001. Antiviral effect of red microalgal polysaccharides on herpes simplex and Varicella zoster viruses. J. Appl. Phycol., 13(2):127-134.

[17] Kneyber, M.C.J., Moll, H.A., de Groot, R., 2000. Treatment and prevention of respiratory syncytial virus infection. Eur. J. Pediatr., 159(6):399-411.

[18] Li, Y.L., But, P.P.H., Ooi, V.E.C., 2005. Antiviral activity and mode of action of caffeoylquinic acids from Schefflera heptaphylla (L.) Frodin. Antiviral Res., 68(1):1-9.

[19] Mayer, A.M.S., Lehmann, V.K.B., 2000. Marine pharmacology in 1998: marine compounds with antibacterial, anticoagulant, antifungal, anti-inflammatory, anthelmintic, antiplatelet, antiprotozoal, and antiviral activities; with actions on the cardiovascular, endocrine, immune, and nervous systems, and other miscellaneous mechanism of action. The Pharmacologist, 42:62-69.

[20] Nakashima, H., Kido, Y., Kobayashi, N., Motoki, Y., Neushul, M., Yamamoto, N., 1987. Prification and characterization of an avian myeloblastosis and human immunodeficiency virus reverse transcriptase inhibitor, sulfated polysaccharides extracted from sea algae. Antimicrob. Agents Chemother., 31(10):1524-1528.

[21] Ponce, N.M.A, Pujol, C.A., Damonte, E.B., Flores, M.L., Stortz, C.A., 2003. Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies. Carbohydr. Res., 338(2):153-165.

[22] Scott, P.D., Ochola, R., Sande, D., Ngama, M., Okiro, E.A., Medley, G.F., Nokes, D.J., Cane, P.A., 2007. Comparison of strain-specific antibody responese during primary and secondary infections with respiratory syncytial virus. J. Med. Virol., 79(12):1943-1950.

[23] Serkedjieva, J., Ivancheva, S., 1998. Antiherpes virus activity of extracts from the medicinal plant Geranium sanguineum L. J. Ethnopharmacol., 64(1):59-68.

[24] Wang, H., Li, Y.L., Shen, W.Z., Rui, W., Ma, X.J., Cen, Y.Z., 2007a. Antiviral activity of a sulfoquinovosyldiacylglycerol (SQDG) compound isolated from the green alga Caulerpa racemosa. Botanica Marina, 50(3):185-190.

[25] Wang, H., Ooi, E.C.V., Ang, P.O.Jr., 2007b. Antiviral polysaccharides isolated from Hong Kong brown seaweed Hydroclathrus clathratus. Sci. China C Life Sci., 50(5):611-618.

[26] Witvrouw, M., de Clercq, E., 1997. Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. Gen. Pharmacol., 29(4):497-511.

[27] Zhu, W., 2002. Antiviral Activities of Selected Hong Kong Marine Algae against Herpes Simplex Viruses and Other Viruses and Their Possible Antiviral Mechanisms. PhD Thesis, the Chinese University of Hong Kong, Hong Kong, China, p.186-188.

[28] Zhu, W., Ooi, V.E.C., Chan, P.K.S., Ang, P.O.Jr., 2003. Isolation and characterization of a sulfated polysaccharide from the brown alga Sargassum patens and determination of its anti-herpes activity. Biochem. Cell Biol., 81(1):25-33.

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oudingqiang<mwlyp@126.com>

2010-08-09 13:12:27

this artical is good

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