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CLC number: S852.35; R363.2

On-line Access: 2017-01-03

Received: 2016-11-03

Revision Accepted: 2016-11-28

Crosschecked: 2016-12-13

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Xun Tan


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.1 P.59-69


Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response

Author(s):  Xun Tan, Fan-guo Juan, Ali Q. Shah

Affiliation(s):  Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   tanxun@zju.edu.cn

Key Words:  Plexiform lesions, Endothelial progenitor cells, Immune response, Hepatocyte growth factor, Broiler chicken

Xun Tan, Fan-guo Juan, Ali Q. Shah. Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response[J]. Journal of Zhejiang University Science B, 2017, 18(1): 59-69.

@article{title="Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response",
author="Xun Tan, Fan-guo Juan, Ali Q. Shah",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response
%A Xun Tan
%A Fan-guo Juan
%A Ali Q. Shah
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 1
%P 59-69
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600500

T1 - Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response
A1 - Xun Tan
A1 - Fan-guo Juan
A1 - Ali Q. Shah
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 1
SP - 59
EP - 69
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600500

plexiform lesions (PLs), which are often accompanied by perivascular infiltrates of mononuclear cells, represent the hallmark lesions of pulmonary arteries in humans suffering from severe pulmonary arterial hypertension (PAH). endothelial progenitor cells (EPCs) have been recently implicated in the formation of PLs in human patients. PLs rarely develop in rodent animal models of PAH but can develop spontaneously in broiler chickens. The aim of the present study was to confirm the presence of EPCs in the PLs in broilers. The immune mechanisms involved in EPC dysfunction were also evaluated. Lungs were collected from commercial broilers at 1 to 4 weeks of age. The right/total ventricle ratios indicated normal pulmonary arterial pressures for all sampled birds. Immunohistochemistry was performed to determine the expressions of EPC markers (CD133 and VEGFR-2) and proangiogenic molecule hepatocyte growth factor (HGF) in the lung samples. An EPC/lymphocyte co-culture system was used to investigate the functional changes of EPCs under the challenge of immune cells. PLs with different cellular composition were detected in the lungs of broilers regardless of age, and they were commonly surrounded by moderate to dense perivascular mononuclear cell infiltrates. Immunohistochemical analyses revealed the presence of CD133+ and VEGFR-2+ cells in PLs. These structures also exhibited a strong expression of HGF. Lymphocyte co-culture enhanced EPC apoptosis and completely blocked HGF-stimulated EPC survival and in vitro tube formation. Taken together, this work provides evidence for the involvement of EPCs in the development of PLs in broilers. It is suggested that the local immune cell infiltrate might serve as a contributor to EPC dysfunction by inducing EPC death and limiting their response to angiogenic stimuli. broiler chickens may be valuable for investigating reversibility of plexogenic arteriopathy using gene-modified inflammation-resistant EPCs.


方法:采集1~4周龄肉鸡肺组织,常规石蜡切片,观察肺血管丛样病变的形成情况;采用免疫组化法检测EPCs表面标志CD133和VEGFR-2的表达以及肝细胞生长因子(HGF)的表达;分离培养晚期EPCs,建立EPCs/淋巴细胞共培养体系,并在共培养体系中添加20 ng/ml HGF,观察EPCs增殖、凋亡和体外管样结构形成的变化。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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