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Yun WU


Ziming REN


Yiping XIA


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.6 P.476-491


Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, Lilium brownii var. giganteum

Author(s):  Yun WU, Minyi SUN, Shiqi LI, Ruihan MIN, Cong GAO, Qundan LYU, Ziming REN, Yiping XIA

Affiliation(s):  Department of Landscape Architecture, School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China; more

Corresponding email(s):   ypxia@zju.edu.cn, zimingren@zju.edu.cn

Key Words:  Lilium, Starch synthesis, Bulblet development, Single nucleotide polymorphism (SNP), Expression pattern

Yun WU, Minyi SUN, Shiqi LI, Ruihan MIN, Cong GAO, Qundan LYU, Ziming REN, Yiping XIA. Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, Lilium brownii var. giganteum[J]. Journal of Zhejiang University Science B, 2021, 22(6): 476-491.

@article{title="Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, Lilium brownii var. giganteum",
author="Yun WU, Minyi SUN, Shiqi LI, Ruihan MIN, Cong GAO, Qundan LYU, Ziming REN, Yiping XIA",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, Lilium brownii var. giganteum
%A Yun WU
%A Minyi SUN
%A Shiqi LI
%A Ruihan MIN
%A Cong GAO
%A Qundan LYU
%A Ziming REN
%A Yiping XIA
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 6
%P 476-491
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000545

T1 - Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, Lilium brownii var. giganteum
A1 - Yun WU
A1 - Minyi SUN
A1 - Shiqi LI
A1 - Ruihan MIN
A1 - Cong GAO
A1 - Qundan LYU
A1 - Ziming REN
A1 - Yiping XIA
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 6
SP - 476
EP - 491
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000545

Starch is the predominant compound in bulb scales, and previous studies have shown that bulblet development is closely associated with starch enrichment. However, how starch synthesis affects bulbification at the molecular level is unclear. In this study, we demonstrate that Lilium brownii var. giganteum, a wild lily with a giant bulb in nature, and L. brownii, the native species, have different starch levels and characteristics according to cytological and ultra-structural observations. We cloned the complete sequence of three key gene-encoding enzymes (LbgAGPS, LbgGBSS, and LbgSSIII) during starch synthesis by rapid amplification of 5' and 3' complementary DNA (cDNA) ends (RACE) technology. Bioinformatics analysis revealed that the proteins deduced by these genes contain the canonical conserved domains. Constructed phylogenetic trees confirmed the evolutionary relationships with proteins from other species, including monocotyledons and dicotyledons. The transcript levels of various tissues and time course samples obtained during bulblet development uncovered relatively high expression levels in bulblets and gradual increase expression accompanying bulblet growth. Moreover, a set of single nucleotide polymorphisms (SNPs) was discovered in the AGPS genes of four lily genotypes, and a purifying selection fashion was predicted according to the non-synonymous/synonymous (Ka/Ks) values. Taken together, our results suggested that key starch-synthesizing genes might play important roles in bulblet development and lead to distinctive phenotypes in bulblet size.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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