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On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2023-11-15

Cited: 0

Clicked: 1052

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xiaogang XU

https://orcid.org/0000-0003-4364-5856

Genxiang MAO

https://orcid.org/0000-0001-7061-4479

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.11 P.1047-1052

http://doi.org/10.1631/jzus.B2300087


Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells


Author(s):  Xuqiang ZHOU, Weihua JIN, Di JIANG, Yipeng XU, Sanying WANG, Xinna WU, Yunchuang CHANG, Huili SU, Tianjun ZHU, Xiaogang XU, Genxiang MAO

Affiliation(s):  College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China; more

Corresponding email(s):   xuxg@zju.edu.cn, maogenxiang@163.com

Key Words:  Senescent cell, Human embryonic lung diploid fibroblast cells, Fucoidan sulfate, AXL, Gene expression


Xuqiang ZHOU, Weihua JIN, Di JIANG, Yipeng XU, Sanying WANG, Xinna WU, Yunchuang CHANG, Huili SU, Tianjun ZHU, Xiaogang XU, Genxiang MAO. Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells[J]. Journal of Zhejiang University Science B, 2023, 24(11): 1047-1052.

@article{title="Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells",
author="Xuqiang ZHOU, Weihua JIN, Di JIANG, Yipeng XU, Sanying WANG, Xinna WU, Yunchuang CHANG, Huili SU, Tianjun ZHU, Xiaogang XU, Genxiang MAO",
journal="Journal of Zhejiang University Science B",
volume="24",
number="11",
pages="1047-1052",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300087"
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%0 Journal Article
%T Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells
%A Xuqiang ZHOU
%A Weihua JIN
%A Di JIANG
%A Yipeng XU
%A Sanying WANG
%A Xinna WU
%A Yunchuang CHANG
%A Huili SU
%A Tianjun ZHU
%A Xiaogang XU
%A Genxiang MAO
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 11
%P 1047-1052
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300087

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T1 - Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells
A1 - Xuqiang ZHOU
A1 - Weihua JIN
A1 - Di JIANG
A1 - Yipeng XU
A1 - Sanying WANG
A1 - Xinna WU
A1 - Yunchuang CHANG
A1 - Huili SU
A1 - Tianjun ZHU
A1 - Xiaogang XU
A1 - Genxiang MAO
J0 - Journal of Zhejiang University Science B
VL - 24
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SP - 1047
EP - 1052
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2300087


Abstract: 
Coronavirus disease 2019 (COVID-19) is a global infectious disease that seriously endangers human life and health and affects normal social activities. Since the pandemic outbreak from December 2019 to February 2023, the total number of confirmed cases has exceeded 753 million, and the deaths caused by COVID-19 have reached 6.6 million (https://covid19.who.int; accessed on Feb. 16, 2023). Currently, supportive therapy is the primary treatment for patients, as some antiviral drugs have demonstrated significant side effects during clinical treatment. Although vaccination has a particular effect on the prevention of novel coronavirus infection and the emergence of severe diseases, the variation in the virus strain caused the vaccine’s protective effect to be greatly weakened or lost. Therefore, there is an urgent need to identify safe and effective drugs to prevent and cure COVID-19. Relevant research and exploration can also provide a reference for drug screening with which to address similar infectious diseases and public health events in the future.

羊栖菜来源的褐藻多糖硫酸酯调控人胚肺二倍体成纤维细胞中新冠病毒受体分子AXL的表达

周叙强1,金维华3,江迪3,徐一鹏4,王三应2,吴辛娜5,常允闯6,苏慧丽2,朱田君2,徐小刚2,毛根祥1
1浙江中医药大学生命科学学院,中国杭州市,310053
2浙江医院,浙江省老年医学重点实验室和浙江省老年医学研究所,中国杭州市,310030
3浙江工业大学生物工程学院,中国杭州市,310014
4浙江省肿瘤医院,浙江省核酸适体与临床诊治重点实验室,中国科学院基础医学与肿瘤研究所,中国杭州市,310022
5浙江大学医学院,中国杭州市,310058
6浙江科技学院生物与化学工程学院,中国杭州市,310023
摘要:新冠病毒感染疫情严重威胁着世界各国人民的生命健康。目前,对病毒感染的防治研究主要集中在抑制病毒与分子受体的结合上。AXL作为新发现的严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)受体,在协助病毒感染人体呼吸系统中发挥着重要作用,是未来临床干预的潜在靶点。本研究对已发表的单细胞测序数据进行整理和分析,发现AXL在年轻人肺细胞中的表达水平明显高于老年人。人胚肺二倍体成纤维细胞(2BS)是衰老研究的公认细胞株。本文采用2BS细胞构建复制性细胞衰老模型,发现年轻细胞中AXL的蛋白水平明显高于衰老细胞,据此推测年轻人感染的风险可能更高,需要注意防护。我们发现一种羊栖菜褐藻多糖硫酸酯组分(SFW-3)可显著下调年轻2BS细胞中AXL的表达水平,表明SFW-3具有一定的抗SARS-CoV-2感染的研究价值,同时表明2BS细胞株也可作为潜在的SARS-CoV-2体外感染模型。

关键词:新冠病毒;褐藻多糖硫酸酯;酪氨酸蛋白激酶受体;血管紧张素转化酶2;年龄

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]BarataPC, RiniBI, 2017. Treatment of renal cell carcinoma: current status and future directions. CA Cancer J Clin, 67(6):507-524.

[2]BéressA, WassermannO, BruhnT, et al., 1993. A new procedure for the isolation of anti-HIV compounds (polysaccharides and polyphenols) from the marine alga fucus vesiculosus. J Nat Prod, 56(4):478-488.

[3]BoehmerTK, DeViesJ, CarusoE, et al., 2020. Changing age distribution of the COVID-19 pandemic—‍United States, May‍–‍August 2020. MMWR Morb Mort Wkly Rep, 69(39):1404-1409.

[4]BuckCB, ThompsonCD, RobertsJN, et al., 2006. Carrageenan is a potent inhibitor of papillomavirus infection. PLoS Pathog, 2(7):e69.

[5]ChoiEM, KimAJ, KimYO, et al., 2005. Immunomodulating activity of arabinogalactan and fucoidan in vitro. J Med Food, 8(4):446-453.

[6]DamonteEB, MatulewiczMC, CerezoAS, 2004. Sulfated seaweed polysaccharides as antiviral agents. Curr Med Chem, 11(18):2399-2419.

[7]FeneyrollesC, SpenlinhauerA, GuietL, et al., 2014. Axl kinase as a key target for oncology: focus on small molecule inhibitors. Mol Cancer Ther, 13(9):2141-2148.

[8]HoshinoT, HayashiT, HayashiK, et al., 1998. An antivirally active sulfated polysaccharide from Sargassum horneri (TURNER) C.AGARDH. Biol Pharm Bull, 21(7):‍730-734.

[9]JinWH, TangH, ZhangJM, et al., 2020. Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity. Int J Biol Macromol, 149:450-458.

[10]LiJH, ZhangZY, TongTJ, 1995. The proliferative response and anti-oncogene expression in old 2BS cells after growth factor stimulation. Mech Ageing Dev, 80(1):25-34.

[11]LimS, ZhangM, ChangTL, 2022. ACE2-independent alternative receptors for SARS-CoV-2. Viruses, 14(11):2535.

[12]LukassenS, ChuaRL, TrefzerT, et al., 2020. SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells. EMBO J, 39(10):e105114.

[13]MaoGX, WangY, QiuQ, et al., 2010. Salidroside protects human fibroblast cells from premature senescence induced by H2O2 partly through modulating oxidative status. Mech Ageing Dev, 131(11-12):723-731.

[14]MoralesA, Rojo RelloS, CristóbalH, et al., 2021. Growth arrest-specific factor 6 (GAS6) is increased in COVID-19 patients and predicts clinical outcome. Biomedicines, 9(4):335.

[15]QueirozKCS, MedeirosVP, QueirozLS, et al., 2008. Inhibition of reverse transcriptase activity of HIV by polysaccharides of brown algae. Biomed Pharmacother, 62(5):303-307.

[16]ReyfmanPA, WalterJM, JoshiN, et al., 2019. Single-cell transcriptomic analysis of human lung provides insights into the pathobiology of pulmonary fibrosis. Am J Respir Crit Care Med, 199(12):1517-1536.

[17]SungnakW, HuangN, BécavinC, et al., 2020. SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes. Nat Med, 26(5):‍681-687.

[18]TangZ, ZhangZ, ZhengY, et al., 1994. Cell aging of human diploid fibroblasts is associated with changes in responsiveness to epidermal growth factor and changes in HER-2 expression. Mech Ageing Dev, 73(1):57-67.

[19]UndurragaEA, ChowellG, MizumotoK, 2021. COVID-19 case fatality risk by age and gender in a high testing setting in Latin America: Chile, March‍–‍August 2020. Inf Dis Poverty, 10:11.

[20]ValverdeP, 2005. Effects of Gas6 and hydrogen peroxide in Axl ubiquitination and downregulation. Biochem Biophys Res Commun, 333(1):180-185.

[21]WangS, QiuZY, HouYN, et al., 2021. AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells. Cell Res, 31(2):‍‍126-140.

[22]WangSY, XuXG, SunC, et al., 2021. Sulphated glucuronomannan tetramer and hexamer from Sargassum thunbergii exhibit anti-human cytomegalovirus activity by blocking viral entry. Carbohyd Polym, 273:118510.

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