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On-line Access: 2024-08-27
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https://orcid.org/0000-0003-3035-278X
Zhijing LIU, Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU. Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances[J]. Journal of Zhejiang University Science B, 2021, 22(7): 533-547.
@article{title="Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances",
author="Zhijing LIU, Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU",
journal="Journal of Zhejiang University Science B",
volume="22",
number="7",
pages="533-547",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000602"
}
%0 Journal Article
%T Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances
%A Zhijing LIU
%A Cong XU
%A Ran TIAN
%A Wan WANG
%A Jiage MA
%A Liya GU
%A Fei LIU
%A Zhanmei JIANG
%A Juncai HOU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 7
%P 533-547
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000602
TY - JOUR
T1 - Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances
A1 - Zhijing LIU
A1 - Cong XU
A1 - Ran TIAN
A1 - Wan WANG
A1 - Jiage MA
A1 - Liya GU
A1 - Fei LIU
A1 - Zhanmei JIANG
A1 - Juncai HOU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 7
SP - 533
EP - 547
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000602
Abstract: Lactic acid bacteria (LAB) are a representative probiotic. As the dominant flora in the human intestinal tract, LAB can regulate the balance of human intestinal flora and improve host health. The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population, with excellent probiotics and high antibacterial activity. After 16S ribosomal RNA (rRNA) homology and phylogenetic tree analysis, potential probiotics were tested for their antibacterial activity, resistance to artificial gastrointestinal fluid and drugs, surface hydrophobicity, and safety. Three strains of LAB with acid resistance, bile salt resistance, epithelial cell adhesion, and no multidrug resistance were selected: Lactobacillus salivarius, Leuconostoc lactis, and Lactobacillus paracasei. Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of L. lactis were organic acids, whereas those of L. salivarius and L. paracasei were organic acids and bacteriocins with broad-spectrum antibacterial activity. These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens.
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